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KD-Validated Anti-Phospho-4E BP1 (Thr46) Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1815
Application
WB, FC, ICC
Uniprot Id
Q13541
Reactivity
Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-phospho-4E BP1 (Thr46) antibody (Cat#ABG1815). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-4E BP1 (Thr46) antibody (Cat#ABG1815, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-phospho-4E BP1 (Thr46) antibody (Cat#ABG1815). Phospho-4E BP1 (Thr46) expression in wild-type (WT) and EIF4EBP1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-phospho-4E BP1 (Thr46) antibody (Cat#ABG1815, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of phospho-4E BP1 (Thr46) expression in C2C12 cells using anti-phospho-4E BP1 (Thr46) antibody (Cat#ABG1815, 1:2,000). Green, isotype control; red, phospho-4E BP1 (Thr46).
  • Immunocytochemical staining of C2C12 cells with anti-Phospho-4E BP1 (Thr46) antibody (Cat#ABG1815, 1:1,000). Nuclei were stained blue with DAPI; Phospho-4E BP1 (Thr46) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
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ABG1815-100 In Stock 100 µL $499.00
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ABG1815-20 In Stock 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Phospho-4E BP1 (Thr46) Rabbit Monoclonal Antibody
Accession (Primary)Q13541
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB4405
Immunogen
A synthesized peptide derived from human Phospho-4E BP1 (Thr46)
Species Reactivity
Reacts WithRat
Calculated MW
Predicted, 13 kDa, observed, 15-20 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q13541 )

NameEIF4EBP1
Other Names
EIF4EBP1; Eukaryotic Translation Initiation Factor 4E Binding Protein 1; PHAS-I ; 4E-BP1; Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1; Eukaryotic Translation Initiation Factor 4E-Binding Protein 1; EIF4E-Binding Protein 1; 4EBP1; BP-1
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