Modification-state-specific Services

Phospho-specific antibodies are critical tools used for research of signaling pathways and regulatory networks in various cellular processes. We have vast experience in the development of custom and off the shelf antibodies against specific phosphorylated residues in different protein targets.

Additionally, We can also develop polyclonal /monoclonal antibodies against methylated and acetylated residues, valuable tools in epigenetics research. Our project managers will work with you from antigen design to validation.

Our Modification-state-specific antibody offering includes:

Antigen design and preparation:	Days
Modified peptide synthesis	7
Peptide conjugation to KLH	14
Control non modified peptide synthesis	7
Peptide conjugation to KLH	14
Animal immunization: rabbits for polyclonal and mouse for monoclonal	1
Test bleed ELISA screening for phosphopeptide, non-phosphopeptide	35-49
Hybridoma fusion and screening for phosphopeptide, non-phosphopeptide clones, clones amplification and storage	50
Ascites production	85
Two step phosho-specific purification by peptide affinity	99
Validation test by WB (limited guarantee available)	3
Validation test by IF (limited guarantee available)	3
Validation by other tests (limited guarantee available)	3-10
Total:	~120-140

Deliverables:

• 2 x 1 ml pre-immune bleeds
• 2 x 1 ml 1st post-immune bleeds
• 10 mg of peptide, 2-10 mg of Ig fraction