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The highly experienced ADMEbio team offers Monoclonal Antibody Service in Balb/c mice. In addition to delivery of purified antibody and hybridoma, accompanying options include antibody characterization in your application, and scale up. Please contact us to learn more about these and other service offerings.
Customizable according to customer requirements
| Antigen design and preparation: | |
| Peptide synthesis of up to 18 AA residues (20-30 mg) with a purity > 80% | |
| Peptide conjugation to KLH |
| Antigen design and preparation: | |
| Recombinant protein preparation | |
| One primary subcutaneous injection of 100 ug conjugated peptide/protein in Complete Freund's Adjuvant | |
| Five subcutaneous injections of 500 ug conjugated peptide/protein in Incomplete Freund's Adjuvant | |
| ELISA analysis on bleed between each intraperitoneal injection | |
| Hybridoma fusion and 2 rounds subcloning including subtype identification and clone amplification | |
| Ascites production | |
| Protein G purification | |
| Validation test by WB (limited guarantee available) | |
| Validation test by IF (limited guarantee available) | |
| Validation by IHC (limited guarantee available) | |
| Validation by FC (limited guarantee available) |
