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ADMEbio is a leading manufacturer of off the shelf and custom antibodies with over 20,000 antibodies developed over the last decade. Our polyclonal antibody services provide a fast and economical solution with extreme flexibility - we offer primary Polyclonal Antibody Service (see list below ) to choose from, where you can provide your own immunogen or work with our world-class synthetic peptide chemistry group to design an epitope-specific and immunogenic peptide to meet your specifications.
| Antigen design and preparation: | Days |
| Peptide synthesis of up to 18 AA residues (20-30 mg) with a purity > 80% | 7 |
| Peptide conjugation to KLH | 14 |
| Antigen design and preparation: | |
| Recombinant protein preparation | 7-14 |
| One primary subcutaneous injection of 200 ug conjugated peptide/protein in Complete Freund's Adjuvant | 1 |
| Five subcutaneous injections of 100 ug conjugated peptide/protein in Incomplete Freund’s Adjuvant | 14-56 |
| Test bleeds and ELISA analysis on test bleed 1, and test bleed 2 if 1 is negative | 35-49 |
| Five production bleeds | 39-69 |
| Terminal bleed by exsanguination (±40 ml serum/rabbit), final ELISA analysis | 85 |
| Protein A and peptide affinity purification for total yield of 2-10 mg IgG from 2 rabbits | 99 |
| Validation test by WB (limited guarantee available) | 3 |
| Validation test by IF (limited guarantee available) | 3 |
| Validation by IHC (limited guarantee available) | 3 |
| Validation by FC (limited guarantee available) | 3 |
| Total: | ~80-100 |
In order to minimize your expense, we offers customized package (see example below):
