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KD-Validated Anti-A-kinase anchoring protein 8 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1158
Application
WB, FC, ICC
Uniprot Id
O43823
Reactivity
Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-A-kinase anchoring protein 8 antibody (Cat#ABG1158). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-A-kinase anchoring protein 8 antibody (Cat#ABG1158, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-A-kinase anchoring protein 8 antibody (Cat#ABG1158). A-kinase anchoring protein 8 expression in wild type (WT) and A-kinase anchoring protein 8 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-A-kinase anchoring protein 8 antibody (Cat#ABG1158, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of A-kinase anchoring protein 8 expression in C2C12 cells using A-kinase anchoring protein 8 antibody (Cat#ABG1158, 1:2,000). Green, isotype control; red, A-kinase anchoring protein 8.
  • Immunocytochemical staining of C2C12 cells A-kinase anchoring protein 8 antibody (Cat#ABG1158, 1:1,000). Nuclei were stained blue with DAPI; A-kinase anchoring protein 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
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ABG1158-100 In Stock 100 µL $499.00
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ABG1158-20 In Stock 20 µL $149.00
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Product Overview

NameKD-Validated Anti-A-kinase anchoring protein 8 Rabbit Monoclonal Antibody
Accession (Primary)O43823
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 2970
Immunogen
A synthesized peptide derived from AKAP 95
Species Reactivity
Reacts WithRat
Calculated MW
Predicted, 76 kDa , observed, 95 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( O43823 )

NameAKAP8
Other Names
AKAP8; A-Kinase Anchoring Protein 8; AKAP95; A Kinase (PRKA) Anchor Protein 8; A-Kinase Anchor Protein, 95kDa; A-Kinase Anchor Protein 8; AKAP 95; AKAP-8; A-Kinase Anchor Protein 95 KDa; DKFZp586B1222; DKFZP586B1222; AKAP-95
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