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KD-Validated Anti-14-3-3 gamma Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1013
Application
WB, FC, ICC
Uniprot Id
P61981
Reactivity
Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-14-3-3 gamma antibody (Cat#ABG1013). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-14-3-3 gamma antibody (Cat#ABG1013, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-14-3-3 gamma antibody (Cat#ABG1013). 14-3-3 gamma expression in wild type (WT) and 14-3-3 gamma shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-14-3-3 gamma antibody (Cat#ABG1013, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of 14-3-3 gamma expression in HepG2 cells using 14-3-3 gamma antibody (Cat#ABG1013, 1:2,000). Green, isotype control; red, 14-3-3 gamma.
  • Immunocytochemical staining of HepG2 cells with 14-3-3 gamma antibody (Cat#ABG1013, 1:1,000). Nuclei were stained blue with DAPI; 14-3-3 gamma was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
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ABG1013-100 In Stock 100 µL $499.00
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ABG1013-20 In Stock 20 µL $149.00
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Product Overview

NameKD-Validated Anti-14-3-3 gamma Rabbit Monoclonal Antibody
Accession (Primary)P61981
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 1970
Immunogen
A synthesized peptide derived from human 14-3-3 gamma
Species Reactivity
Reacts WithRat
Calculated MW
Predicted, 28 kDa , observed, 28 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P61981 )

NameYWHAG
Other Names
YWHAG; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Gamma; 14-3-3GAMMA; PPP1R170; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Gamma Polypeptide; Protein Phosphatase 1, Regulatory Subunit 170; Protein Kinase C Inhibitor Protein 1; 14-3-3 Protein Gamma; KCIP-1; 14-3-3 Gamma; 14-3-3γ; 14-3-3G; EIEE56; DEE56
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