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KD-Validated Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1643
Application
WB, FC, ICC
Uniprot Id
P31749
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Phospho-AKT1 (S124) antibody (Cat#ABG1643). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-AKT1 (S124) antibody (Cat#ABG1643, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-phospho-AKT1 (S124) antibody (Cat#ABG1643). Phospho-AKT1 (S124) expression in wild-type (WT) and AKT1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-AKT1 (S124) antibody (Cat#ABG1643, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Phospho-AKT1 (S124) expression in C2C12 cells using Phospho-AKT1 (S124) antibody (Cat#ABG1643, 1:2,000). Green, isotype control; red, Phospho-AKT1 (S124).
  • Immunocytochemical staining of C2C12 cells with Phospho-AKT1 (S124) antibody (Cat#ABG1643, 1:1,000). Nuclei were stained blue with DAPI; Phospho-AKT1 (S124) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1643-100 3 days 100 µL $499.00
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ABG1643-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Phospho-AKT1 (S124) Rabbit Monoclonal Antibody
Accession (Primary)P31749
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB1180
Immunogen
A synthesized peptide derived from human AKT1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 56 kDa , observed, 60 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P31749 )

Gene NameAKT1
Other Gene Names
AKT1; AKT Serine/Threonine Kinase 1; RAC; PKB; PRKBA; AKT; V-Akt Murine Thymoma Viral Oncogene Homolog 1; RAC-Alpha Serine/Threonine-Protein Kinase; Protein Kinase B Alpha; Proto-Oncogene C-Akt; Protein Kinase B; RAC-PK-Alpha; EC 2.7.11.1; RAC-ALPHA; PKB Alpha; V-Akt Murine Thymoma Viral Oncogene-Like Protein 1; Serine-Threonine Protein Kinase; Rac Protein Kinase Alpha; RAC-Alpha; PKB-ALPHA; EC 2.7.11; AKT1m
About Gene Symbol
AKT1 (AKT Serine/Threonine Kinase 1) is a serine/threonine protein kinase involved in cell signaling and proliferation. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic t
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