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KD-Validated Anti-ABCB1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1002
Application
WB, ICC
Uniprot Id
P08183
Reactivity
Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-ABCB1 antibody (Cat#ABG1002). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ABCB1 antibody (Cat#ABG1002, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-ABCB1 antibody (Cat#ABG1002). ABCB1 expression in wild type (WT) and ABCB1 shRNA knockdown (KD) Hela cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ABCB1 antibody (Cat#ABG1002, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Immunocytochemical staining of HepG2 cells with ABCB1 antibody (Cat#ABG1002, 1:1,000). Nuclei were stained blue with DAPI; ABCB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
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ABG1002-100 In Stock 100 µL $499.00
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ABG1002-20 In Stock 20 µL $149.00
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Product Overview

NameKD-Validated Anti-ABCB1 Rabbit Monoclonal Antibody
Accession (Primary)P08183
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB1330
Immunogen
A synthesized peptide derived from human P Glycoprotein 1
Species Reactivity
Reacts WithRat
Calculated MW
Predicted, 141 kDa , observed, 130-180 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:00-1:1,000

Target Information: ( P08183 )

NameABCB1
Other Names
ABCB1; ATP Binding Cassette Subfamily B Member 1; PGY1; Multidrug Resistance Protein 1; CD243; GP170; ABC20; P-170; MDR1; ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1; ATP-Dependent Translocase ABCB1; Phospholipid Transporter ABCB1; Colchicin Sensitivity; P-Glycoprotein 1; CLCS; P-GP; ATP-Binding Cassette Sub-Family B Member 1; Doxorubicin Resistance; P-Glycoprotein; CD243 Antigen; EC 3.6.3.44; EC 7.6.2.2; EC 7.6.2.1; EC 3.6.3; P-Gp
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