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HBsAgA Antibody

Hepatitis B surface Antigen A, Polyclonal Goat Antibody

 
Catalog #
ABP0013
 
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ABP0013-1 7 days 1 ml $280.00
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ABP0013-5 7 days 5 ml $1,400.00
ABP0013-10 7 days 10 ml $1,920.00
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Product Overview

NameHBsAgA Antibody
Description
Hepatitis B surface Antigen A, Polyclonal Goat Antibody
Immunogen
r.Hep B vaccine (BTG).
Introduction
Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA (pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA.
Formulation
1mg/ml in PBS (after reconstitution).
Solubility
Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.
Applications
Direct ELISA, Western Blot, Immuneprecipitation, immunohistochemistry.
Type
Mouse antibody Monoclonal.
Clone
NYRHepB-1.
Ig Subclass
mouse IgG2b.
Purification Method
Ion exchange column.
Storage Procedures
In lyophilized form, for long periods, store at 4°C in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20°C.
Shipping Conditions
Antibody is shipped lyophilized at ambient temperature.
Titer
By direct ELISA (against r.hepatitis vaccine), 1:10,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).
Note
This antibody recognizes both the AD and the AY antigens in direct ELISA and works in capture ELISA with anti-hepatitis Ag-2.
Precautions
HBsAgA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
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